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resource source identifier antibodies hla class i abc rabbit polyclonal antibody proteintech  (Proteintech)


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    Structured Review

    Proteintech resource source identifier antibodies hla class i abc rabbit polyclonal antibody proteintech
    Resource Source Identifier Antibodies Hla Class I Abc Rabbit Polyclonal Antibody Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hla+abc/ABCB6+Fusion+Protein/pm41923622-483-2-13
    Average 94 stars, based on 4 article reviews
    resource source identifier antibodies hla class i abc rabbit polyclonal antibody proteintech - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: CDK4/6 Inhibition Induces CD8 + T Cell Antitumor Immunity via MIF-Induced Functional Orchestration of Tumor-Associated Macrophages.
    Article Snippet: The slices were washed two to three times in washing buffer 2 (0.1% Triton, 0.02% SDS [Sigma; 428015], 0.2% BSA, 50 μg mL−1 ascorbic acid [Sigma; 1043003], 0.05 ngmL−1 L-glutathione [Sigma; G4251] in PBS) on a roller mixer in the dark at 4 °C, with the solution replaced every hour. .. 3D-IF: After fixation with 4% PFA on a roller mixer in the dark at 4 °C for 1 to 2 h, the slices were incubated in washing buffer on a roller mixer in the dark at 4 °C for 1 h. The tissues were further incubated in washing buffer 1 (0.2% Tween-20 [Sigma; P7949], 0.2% Triton X-100, 0.02% SDS, 0.2% BSA, 50 μg mL−1 ascorbic acid, 0.05 ng mL−1 L-glutathione in PBS) on a roller mixer in the dark at 4 °C for 2 to 3 h. The tumor slices were then incubated overnight in primary antibodies (CD8a [Abcam; ab316778 and ab119857], F4/80 [ThermoFisher; MA1-91124], CD86 [Abcam; ab220188], CD206 [Cell Signaling Technology; 24595S], HLA-ABC [Proteintech; 15240- 1-AP], and granzyme B [Cell Signaling Technology; 17215S]), diluted in washing buffer 2 at appropriate concentrations, on a roller mixer in the dark at 4 °C. ..

    Western Blot:

    Article Title: Combined Photothermal and mTOR‐Targeted Therapy Overcomes Immune Evasion and Enhances Checkpoint Blockade Efficacy in Metastatic Triple‐Negative Breast Cancer
    Article Snippet: The resulting protein extracts were subjected to electrophoresis and transferred onto PVDF membranes (Bio‐Rad). .. Western blot analysis was conducted using the following antibodies: phosphorylated mTOR (Cell Signaling, #5536S, RRID: AB_10694663, 1/1000), phosphorylated Akt (S473) (Cell Signaling, #4060L, RRID: AB_2315049, 1/2000), phosphorylated S6 (S240/244) (Cell Signaling, #5364L, RRID: AB_10694233, 1/1000), phosphorylated 4EBP1 (Cell Signaling, #2855L, RRID: AB_560835, 1/1000), HLA‐ABC (Proteintech, #15240‐1‐AP, RRID: AB_2115170, 1/2000), B2M (Abcam, #ab218230, RRID: AB_2891403, 1/1000), phosphorylated p38·MAPK (Cell Signaling, #9216S, RRID:AB_3105021, 1/1000), HIF‐1α (Abcam, #ab228649, RRID:AB_2739388,1/1000), HSP70 (Abcam, #5439, RRID:AB_2650529, 1/1000), HSP27 (Cell Signaling, #50353S, RRID:AB_2572328, 1/1000), Cleaved‐caspase‐3 (Cell Signaling, #9664S, RRID:AB_3713233, 1/1000), CHOP (Proteintech, #15204‐1‐AP, RRID:AB_3714679, 1/1000), phosphorylated elF2α (Cell Signaling, #3398T, RRID:AB_2629818, 1/1000), phosphorylated PERK (Proteintech, #15033, RRID:AB_3103093, 1/1000), actin (ZSbio, #TA‐09, RRID:AB_3716396, 1/1000), and α‐tubulin (Abcam, #ab176560, RRID: AB_2737386, 1/2000). ..

    Article Title: Combined Photothermal and mTOR-Targeted Therapy Overcomes Immune Evasion and Enhances Checkpoint Blockade Efficacy in Metastatic Triple-Negative Breast Cancer.
    Article Snippet: The resulting protein extracts were subjected to electrophoresis and transferred onto PVDF membranes (Bio-Rad). .. Western blot analysis was conducted using the following antibodies: phosphorylated mTOR (Cell Signaling, #5536S, RRID: AB_10694663, 1/1000), phosphorylated Akt (S473) (Cell Signaling, #4060L, RRID: AB_2315049, 1/2000), phosphorylated S6 (S240/244) (Cell Signaling, #5364L, RRID: AB_10694233, 1/1000), phosphorylated 4EBP1 (Cell Signaling, #2855L, RRID: AB_560835, 1/1000), HLA-ABC (Proteintech, #15240-1-AP, RRID: AB_2115170, 1/2000), B2M (Abcam, #ab218230, RRID: AB_2891403, 1/1000), phosphorylated p38·MAPK (Cell Signaling, #9216S, RRID:AB_3105021, 1/1000), HIF-1α (Abcam, #ab228649, RRID:AB_2739388,1/1000), HSP70 (Abcam, #5439, RRID:AB_2650529, 1/1000), HSP27 (Cell Signaling, #50353S, RRID:AB_2572328, 1/1000), Cleaved-caspase-3 (Cell Signaling, #9664S, RRID:AB_3713233, 1/1000), CHOP (Proteintech, #15204-1-AP, RRID:AB_3714679, 1/1000), phosphorylated elF2α (Cell Signaling, #3398T, RRID:AB_2629818, 1/1000), phosphorylated PERK (Proteintech, #15033, RRID:AB_3103093, 1/1000), actin (ZSbio, #TA-09, RRID:AB_3716396, 1/1000), and α-tubulin (Abcam, #ab176560, RRID: AB_2737386, 1/2000). ..

    Immunohistochemistry:

    Article Title: Sequential modulation of the Wnt/β-catenin signaling pathway enhances tumor-intrinsic MHC I expression and tumor clearance
    Article Snippet: .. Immunohistochemistry was performed using HLA-ABC (Proteintech Catalog # 15240–1-AP) antibody with appropriate primary and secondary comparison controls. ..

    Comparison:

    Article Title: Sequential modulation of the Wnt/β-catenin signaling pathway enhances tumor-intrinsic MHC I expression and tumor clearance
    Article Snippet: .. Immunohistochemistry was performed using HLA-ABC (Proteintech Catalog # 15240–1-AP) antibody with appropriate primary and secondary comparison controls. ..



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    (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. (H) Multiplex immunohistochemistry (mIHC) showing <t>CK7</t> + (green) HLA-A/B + (magenta) trophoblasts in VUE tissue (white arrows).
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    (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. <t>(H)</t> <t>Multiplex</t> immunohistochemistry (mIHC) showing CK7 + (green) <t>HLA-A/B</t> + (magenta) trophoblasts in VUE tissue (white arrows).
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    (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. <t>(H)</t> <t>Multiplex</t> immunohistochemistry (mIHC) showing CK7 + (green) <t>HLA-A/B</t> + (magenta) trophoblasts in VUE tissue (white arrows).
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    Image Search Results


    Cytomix increased the expression of immunomodulatory markers on BM-MSCs, iMSCs WT and iMSCs B2M KO. The gating strategy is shown in . ( A ) Histogram charts of the expression of the immunomodulatory markers on BM-MSCs, iMSCs WT and iMSCs B2M KO. ( B – H ) Cytomix significantly increased the expression levels of HLA-ABC on BM-MSCs and iMSCs WT, HLA-DR on BM-MSCs, CD54 on all types of MSCs, CD200 on iMSCs WT, CD119 and CD120b on BM-MSCs, and CD274 on iMSCs WT and iMSCs B2M KO. Representative results of three independent experiments are shown. Results are shown as mean ± SD ( n = 3 in each group) and analysed by two-way ANOVA with Šídák’s multiple comparisons test or Tukey’s multiple comparisons test. */**/***/**** represent comparisons of naïve vs. Cytomix in each type of MSC; $/$$$/$$$$ represent comparisons of BM-MSCs vs. iMSCs WT vs. iMSCs B2M KO in the naïve group; ##/###/#### represent comparisons of BM-MSCs vs. iMSCs WT vs. iMSCs B2M KO in the Cytomix group with significance levels of p < 0.05/0.01/0.001/0.0001, respectively. Nd represents not detectable.

    Journal: International Journal of Molecular Sciences

    Article Title: Proinflammatory Cytokine Preconditioning Enhances the Therapeutic Potency of Different Types of MSCs in Inflammation

    doi: 10.3390/ijms27094090

    Figure Lengend Snippet: Cytomix increased the expression of immunomodulatory markers on BM-MSCs, iMSCs WT and iMSCs B2M KO. The gating strategy is shown in . ( A ) Histogram charts of the expression of the immunomodulatory markers on BM-MSCs, iMSCs WT and iMSCs B2M KO. ( B – H ) Cytomix significantly increased the expression levels of HLA-ABC on BM-MSCs and iMSCs WT, HLA-DR on BM-MSCs, CD54 on all types of MSCs, CD200 on iMSCs WT, CD119 and CD120b on BM-MSCs, and CD274 on iMSCs WT and iMSCs B2M KO. Representative results of three independent experiments are shown. Results are shown as mean ± SD ( n = 3 in each group) and analysed by two-way ANOVA with Šídák’s multiple comparisons test or Tukey’s multiple comparisons test. */**/***/**** represent comparisons of naïve vs. Cytomix in each type of MSC; $/$$$/$$$$ represent comparisons of BM-MSCs vs. iMSCs WT vs. iMSCs B2M KO in the naïve group; ##/###/#### represent comparisons of BM-MSCs vs. iMSCs WT vs. iMSCs B2M KO in the Cytomix group with significance levels of p < 0.05/0.01/0.001/0.0001, respectively. Nd represents not detectable.

    Article Snippet: The cells were centrifuged at 300× g for 5 min and resuspended in 100 μL FACS buffer containing anti-human HLA-ABC-VioGreen, HLA-DR-VioBlue, CD54-APC, CD274-PE-Vio ® 615, CD119-FITC (Cat# 130-120-436, 130-111-794, 130-121-342, 130-122-811, 130-099-931; Miltenyi Biotec, Bergisch Gladbach, Germany), CD120b-PE and CD200-PE-CY7 antibodies (Cat# 358403, 399805; BioLegend, San Diego, CA, USA).

    Techniques: Expressing

    (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. (H) Multiplex immunohistochemistry (mIHC) showing CK7 + (green) HLA-A/B + (magenta) trophoblasts in VUE tissue (white arrows).

    Journal: bioRxiv

    Article Title: Single-cell Analysis of Paired FFPE Placentas Reveals Trophoblast Reprogramming and Immune Dysregulation in Chronic Villitis of Unknown Etiology

    doi: 10.64898/2026.02.28.708431

    Figure Lengend Snippet: (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. (H) Multiplex immunohistochemistry (mIHC) showing CK7 + (green) HLA-A/B + (magenta) trophoblasts in VUE tissue (white arrows).

    Article Snippet: For multiplex immunohistochemical staining, primary antibodies are against human CK7 (17513, Proteintech), and HLA-A/B (15240-1-AP, Proteintech), the Four Detect Kit for Rabbit Primary Antibody kit (PK10033, Proteintech) used for multiple staining.

    Techniques: Expressing, Control, Multiplex Assay, Immunohistochemistry

    (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. (H) Multiplex immunohistochemistry (mIHC) showing CK7 + (green) HLA-A/B + (magenta) trophoblasts in VUE tissue (white arrows).

    Journal: bioRxiv

    Article Title: Single-cell Analysis of Paired FFPE Placentas Reveals Trophoblast Reprogramming and Immune Dysregulation in Chronic Villitis of Unknown Etiology

    doi: 10.64898/2026.02.28.708431

    Figure Lengend Snippet: (A) Expression of MHC class I and II molecules in VUE trophoblasts compared to controls. (B) UMAP visualization of trophoblast re-clustering, identifying 8 distinct subpopulations. (C) Bubble chart of MHC expression across subclusters. (D) Stacked bar plot of relative cellular composition of each trophoblasts cell subset. (E) Box plots of the trophoblasts cell subset proportions between Control and VUE. Red arrows indicate expanded populations, while green arrows indicate depleted populations. (F) Volcano plots of trophoblasts cell subset differentially expressed genes. (G) Gene Set Enrichment analysis of EVT_2 and STB_2. (H) Multiplex immunohistochemistry (mIHC) showing CK7 + (green) HLA-A/B + (magenta) trophoblasts in VUE tissue (white arrows).

    Article Snippet: For multiplex immunohistochemical staining, primary antibodies are against human CK7 (17513, Proteintech), and HLA-A/B (15240-1-AP, Proteintech), the Four Detect Kit for Rabbit Primary Antibody kit (PK10033, Proteintech) used for multiple staining.

    Techniques: Expressing, Control, Multiplex Assay, Immunohistochemistry